Citation Info

Med Sci Sports Exerc. 37(8):1283-1290. Available: www.gssiweb.org

Muscle cytokine mRNA changes after 2.5 h of cycling: influence of carbohydrate.

Nieman DC, Davis JM, Henson DA, Gross SJ, Dumke CL, Utter AC, Vinci DM, Carson JA, Brown A, Mcanulty SR, Mcanulty LS, Triplett NT.

Abstract

PURPOSE: 
To study the effect of carbohydrate compared to placebo ingestion on plasma cytokines and muscle cytokine mRNA following 2.5 h of intensive cycling in 15 trained cyclists.

METHODS:
Fifteen trained cyclists cycled for 2.5 h at 60% Wmax on two occasions while receiving 4 mLkg^-1∑15 min^-1 carbohydrate (6%) (CHO) or placebo (PLA) beverages in a randomized, counterbalanced design. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and 12 h postexercise and compared to samples taken from five cyclists who rested in the lab during the exercise sessions. Blood cell counts were determined, and plasma was analyzed for interleukin (IL)-6, IL-10, IL-1 receptor antagonist (ra), IL-8, cortisol, epinephrine, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of four cytokines, IL-6, IL-8, tumor necrosis factor (TNF) ‚àû, and IL-1Œ≤, using real-time quantitative reverse transcriptase polymerase chain reaction.

RESULTS: 
Plasma glucose and insulin were higher, and epinephrine, cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO versus PLA. Muscle glycogen content decreased 68% immediately postexercise and the pattern of change did not differ between CHO and PLA. Muscle IL-6, IL-8, TNF-‚àû, but not IL-1Œ= mRNA increased immediately postexercise compared to controls, with no differences between CHO and PLA.

CONCLUSIONS:
CHO compared to PLA beverage ingestion attenuated the increase in plasma cortisol, epinephrine, IL-6, IL-10, and IL-1ra, but not muscle IL-6, IL-8, and TNF-‚àû mRNA in athletes cycling 2.5 h at 60% Wmax.

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